Our laboratories are housed in the Multidisciplinary Research Building on the University of Kansas West Campus in Lawrence.
The Main Laboratory is equipped to perform extensive spectroscopic studies including circular dichroism (CD), fluorescence, UV-visible absorbance, and Fourier transform IR (FTIR) measurements over a wide temperature range (0 - 90 °C). Our instrumentation is also designed for high-throughput studies, permitting the rapid accumulation of data. Thermodynamic measurements are performed in high-throughput, high-sensitivity scanning calorimeters, as well as in a high-sensitivity pressure perturbation calorimeter and an ultrasonic spectrometer that greatly expand our capabilities in characterizing molecular stability. Hydrodynamic measurements are performed with high-throughput dynamic light scattering (DLS), high-sensitivity analytical ultracentrifugation (AUC), and high-performance liquid chromatography (HPLC) equipment. Automated generation of empirical phase diagrams (EPDs) is available with the Applied Photophysics Chirascan, the OLIS protein machine, and the Avacta fluorescence system.
A state-of-the-art Biological Safety Level 3 Laboratory fully equipped for macromolecular formulation studies and an adjacent tissue culture facility are an integral part of the laboratory. The state-of-the-art tissue culture facility allows us to perform a range of biological studies, including plaque and various cell-based assays.
The Mass Spectroscopy Laboratory is equipped with Thermo Sceintific LTQ XL mass Spectrometer with Shimadzu HPLC and MALDI-TOF dual detector mass spectrometer. The MS instrument is interfaced to a LC system capable of two-dimensional separations with direct output to the Q-TOF instrument. The Particle Laboratory is equipped with a Brightwell micro-flow imaging flow microscope, a Nanosight particle tracking system, and an FTIR microscope
The lab borders the University's Structural Biology Center, which gives us direct access to other mass spectrometers, including FTMS as well as high field NMRs (600 and 800 MHz). We also have access to modulated DSC and powder x-ray diffraction, as well as electron microscopes.
Additional analytical packages (protein oxidation, glycoprotein characterization, and H/D-MS) are carried out in other laboratories at KU.